Mouse Fractalkine/CX3CL1 ELISA Kit PicoKine®

Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice) . CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 378 415 410 441 411 22.39 5.4% Sample 2 2168 2082 1886 2198 2083 121.71 5.8% Sample 3 9077 9811 9555 9593 9509 267.86 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 378 415 410 441 411 22.39 5.4% Sample 1 378 415 410 441 411 22.39 5.4% Sample 2 2168 2082 1886 2198 2083 121.71 5.8% Sample 2 2168 2082 1886 2198 2083 121.71 5.8% Sample 3 9077 9811 9555 9593 9509 267.86 2.8% Sample 3 9077 9811 9555 9593 9509 267.86 2.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 378 2168 9077 392 1975 9206 Standard deviation 17.76 101.89 653.54 23.52 114.55 810.12 CV (%) 4.7% 4.7% 7.2% 6% 5.8% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 378 2168 9077 392 1975 9206 Standard deviation 17.76 101.89 653.54 23.52 114.55 810.12 CV (%) 4.7% 4.7% 7.2% 6% 5.8% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 378 2168 9077 392 1975 9206 Mean (pg/ml) 378 2168 9077 392 1975 9206 Standard deviation 17.76 101.89 653.54 23.52 114.55 810.12 Standard deviation 17.76 101.89 653.54 23.52 114.55 810.12 CV (%) 4.7% 4.7% 7.2% 6% 5.8% 8.8% CV (%) 4.7% 4.7% 7.2% 6% 5.8% 8.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.074 0.132 0.224 0.423 0.807 1.495 2.33 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.074 0.132 0.224 0.423 0.807 1.495 2.33 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.074 0.132 0.224 0.423 0.807 1.495 2.33 O.D. 0.022 0.074 0.132 0.224 0.423 0.807 1.495 2.33

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.