Human Fractalkine/CX3CL1 ELISA Kit PicoKine®

Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice) . CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 296 303 298 261 289 16.65 5.7% Sample 2 1773 1559 1657 1549 1634 90.41 5.5% Sample 3 5345 5189 5317 5757 5402 213.22 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 296 303 298 261 289 16.65 5.7% Sample 1 296 303 298 261 289 16.65 5.7% Sample 2 1773 1559 1657 1549 1634 90.41 5.5% Sample 2 1773 1559 1657 1549 1634 90.41 5.5% Sample 3 5345 5189 5317 5757 5402 213.22 3.9% Sample 3 5345 5189 5317 5757 5402 213.22 3.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 296 1773 5345 269 1730 5847 Standard deviation 16.87 90.42 315.35 17.75 103.8 374.2 CV (%) 5.7% 6.4% 5.9% 6.6% 6% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 296 1773 5345 269 1730 5847 Standard deviation 16.87 90.42 315.35 17.75 103.8 374.2 CV (%) 5.7% 6.4% 5.9% 6.6% 6% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 296 1773 5345 269 1730 5847 Mean (pg/ml) 296 1773 5345 269 1730 5847 Standard deviation 16.87 90.42 315.35 17.75 103.8 374.2 Standard deviation 16.87 90.42 315.35 17.75 103.8 374.2 CV (%) 5.7% 6.4% 5.9% 6.6% 6% 6.4% CV (%) 5.7% 6.4% 5.9% 6.6% 6% 6.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.112 0.142 0.24 0.43 0.76 1.27 1.871 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.112 0.142 0.24 0.43 0.76 1.27 1.871 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.112 0.142 0.24 0.43 0.76 1.27 1.871 O.D. 0.046 0.112 0.142 0.24 0.43 0.76 1.27 1.871

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.