Mouse Flt-3 Ligand ELISA Kit PicoKine®

Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1) . In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 73 74 70 70 3.11 4.4% Sample 2 243 247 257 224 242 11.96 4.9% Sample 3 884 811 863 831 847 28.18 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 73 74 70 70 3.11 4.4% Sample 1 66 73 74 70 70 3.11 4.4% Sample 2 243 247 257 224 242 11.96 4.9% Sample 2 243 247 257 224 242 11.96 4.9% Sample 3 884 811 863 831 847 28.18 3.3% Sample 3 884 811 863 831 847 28.18 3.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 243 884 69 243 901 Standard deviation 3.3 10.44 59.22 4.27 14.58 64.87 CV (%) 5% 4.3% 6.7% 6.2% 6% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 243 884 69 243 901 Standard deviation 3.3 10.44 59.22 4.27 14.58 64.87 CV (%) 5% 4.3% 6.7% 6.2% 6% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 66 243 884 69 243 901 Mean (pg/ml) 66 243 884 69 243 901 Standard deviation 3.3 10.44 59.22 4.27 14.58 64.87 Standard deviation 3.3 10.44 59.22 4.27 14.58 64.87 CV (%) 5% 4.3% 6.7% 6.2% 6% 7.2% CV (%) 5% 4.3% 6.7% 6.2% 6% 7.2%

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.072 0.155 0.217 0.313 0.449 0.678 1.09 1.834 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.072 0.155 0.217 0.313 0.449 0.678 1.09 1.834 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.072 0.155 0.217 0.313 0.449 0.678 1.09 1.834 O.D. 0.072 0.155 0.217 0.313 0.449 0.678 1.09 1.834

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.