Mouse FASL ELISA Kit PicoKine®

FAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M (r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 68 63 68 59 64 3.77 5.8% Sample 2 206 196 205 225 208 10.55 5% Sample 3 772 915 764 889 835 67.68 8.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 68 63 68 59 64 3.77 5.8% Sample 1 68 63 68 59 64 3.77 5.8% Sample 2 206 196 205 225 208 10.55 5% Sample 2 206 196 205 225 208 10.55 5% Sample 3 772 915 764 889 835 67.68 8.1% Sample 3 772 915 764 889 835 67.68 8.1%

Tumor necrosis factor ligand superfamily member 6; CD95 ligand; CD95-L; Fas antigen ligand; Fas ligand; FasL; CD178; Tumor necrosis factor ligand superfamily member 6, membrane form; Tumor necrosis factor ligand superfamily member 6, soluble form; Receptor-binding FasL ectodomain; Soluble Fas ligand; sFasL; ADAM10-processed FasL form; APL; FasL intracellular domain; FasL ICD; SPPL2A-processed FasL form; SPA; Faslg; Apt1lg1, Cd95l, Fasl, gld, Tnfsf6

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 68 206 772 65 216 727 Standard deviation 2.99 13.59 49.4 3.51 17.28 47.98 CV (%) 4.4% 6.6% 6.4% 5.4% 8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 68 206 772 65 216 727 Standard deviation 2.99 13.59 49.4 3.51 17.28 47.98 CV (%) 4.4% 6.6% 6.4% 5.4% 8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 68 206 772 65 216 727 Mean (pg/ml) 68 206 772 65 216 727 Standard deviation 2.99 13.59 49.4 3.51 17.28 47.98 Standard deviation 2.99 13.59 49.4 3.51 17.28 47.98 CV (%) 4.4% 6.6% 6.4% 5.4% 8% 6.6% CV (%) 4.4% 6.6% 6.4% 5.4% 8% 6.6%

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:7511063) . Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:19794494, PubMed:7532682) . Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (PubMed:19794494) . TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (PubMed:10779162) . Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Isoform FasL: Cell membrane ; Single- pass type II membrane protein . Cytoplasmic vesicle lumen . Lysosome lumen . Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane. .

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.09 0.141 0.258 0.492 0.903 1.634 2.892 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.09 0.141 0.258 0.492 0.903 1.634 2.892 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.09 0.141 0.258 0.492 0.903 1.634 2.892 O.D. 0.023 0.09 0.141 0.258 0.492 0.903 1.634 2.892

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.