Human FAS/Cd95 ELISA Kit PicoKine®

Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF) /tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 68 60 71 60 64 4.86 7.5% Sample 2 235 248 233 262 244 11.62 4.7% Sample 3 915 855 754 803 831 59.88 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 68 60 71 60 64 4.86 7.5% Sample 1 68 60 71 60 64 4.86 7.5% Sample 2 235 248 233 262 244 11.62 4.7% Sample 2 235 248 233 262 244 11.62 4.7% Sample 3 915 855 754 803 831 59.88 7.2% Sample 3 915 855 754 803 831 59.88 7.2%

Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD95; FAS; APT1, FAS1, TNFRSF6

Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. .

Adaptive Immunity, Apoptosis, Autoimmune, Cell Biology, Cytokines, Hematopoietic Progenitors, Immune System Diseases, Immunology, Innate Immunity, Lymphoid, Stem Cells, T Lymphocytic Lineage, TNF Superfamily

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 68 235 915 63 239 861 Standard deviation 3.6 14.8 44.83 3.33 18.88 54.24 CV (%) 5.3% 7.9% 4.9% 5.3% 7.9% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 68 235 915 63 239 861 Standard deviation 3.6 14.8 44.83 3.33 18.88 54.24 CV (%) 5.3% 7.9% 4.9% 5.3% 7.9% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 68 235 915 63 239 861 Mean (pg/ml) 68 235 915 63 239 861 Standard deviation 3.6 14.8 44.83 3.33 18.88 54.24 Standard deviation 3.6 14.8 44.83 3.33 18.88 54.24 CV (%) 5.3% 7.9% 4.9% 5.3% 7.9% 6.3% CV (%) 5.3% 7.9% 4.9% 5.3% 7.9% 6.3%

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.087 0.128 0.163 0.241 0.484 0.748 1.356 2.093 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.087 0.128 0.163 0.241 0.484 0.748 1.356 2.093 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.087 0.128 0.163 0.241 0.484 0.748 1.356 2.093 O.D. 0.087 0.128 0.163 0.241 0.484 0.748 1.356 2.093

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.