Human CCL24/Eotaxin-2 ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 24 (CCL24) also known as myeloid progenitor inhibitory factor 2 (MPIF-2) or eosinophil chemotactic protein 2 (eotaxin-2) is a protein that in humans is encoded by the CCL24 gene. By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23. CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 82 86 80 80 3.96 4.9% Sample 2 209 190 207 207 203 7.69 3.7% Sample 3 857 1006 836 933 908 67.1 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 82 86 80 80 3.96 4.9% Sample 1 75 82 86 80 80 3.96 4.9% Sample 2 209 190 207 207 203 7.69 3.7% Sample 2 209 190 207 207 203 7.69 3.7% Sample 3 857 1006 836 933 908 67.1 7.3% Sample 3 857 1006 836 933 908 67.1 7.3%

C-C motif chemokine 24; CK-beta-6; Eosinophil chemotactic protein 2; Eotaxin-2; Myeloid progenitor inhibitory factor 2; MPIF-2; Small-inducible cytokine A24; CCL24; MPIF2, SCYA24

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 6.3% 5.2% 5.9% 7.2% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 6.3% 5.2% 5.9% 7.2% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 6.3% 5.2% 5.9% 7.2% 5.3% CV (%) 4.9% 6.3% 5.2% 5.9% 7.2% 5.3%

Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.018 0.054 0.095 0.189 0.358 0.759 1.468 2.797 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.018 0.054 0.095 0.189 0.358 0.759 1.468 2.797 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.018 0.054 0.095 0.189 0.358 0.759 1.468 2.797 O.D. 0.018 0.054 0.095 0.189 0.358 0.759 1.468 2.797

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.