Mouse Eotaxin-1 ELISA Kit PicoKine®

Mouse Eotaxin-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ccl11 in cell culture supernatants, serum and plasma (citrate, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 36 38 36 36 1.41 3.9% Sample 2 223 227 217 219 221 3.84 1.7% Sample 3 405 412 415 422 413 6.1 1.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 36 38 36 36 1.41 3.9% Sample 1 34 36 38 36 36 1.41 3.9% Sample 2 223 227 217 219 221 3.84 1.7% Sample 2 223 227 217 219 221 3.84 1.7% Sample 3 405 412 415 422 413 6.1 1.4% Sample 3 405 412 415 422 413 6.1 1.4%

Synonyms

Eotaxin; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11; Ccl11; Scya11

Gene Name

CCL11

Gene ID

20292

UniProt

P48298

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H24-P97

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed constitutively in the thymus. Expression inducible in the lung (type I alveolar epithelial cells), intestine, heart, spleen, kidney.

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (citrate, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 223 405 33 223 429 Standard deviation 1.49 11.59 26.4%2 2.07 12.71 33.46 CV (%) 4.4% 5.2% 6.4% 5.7%% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 223 405 33 223 429 Standard deviation 1.49 11.59 26.4%2 2.07 12.71 33.46 CV (%) 4.4% 5.2% 6.4% 5.7%% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 223 405 33 223 429 Mean (pg/ml) 34 223 405 33 223 429 Standard deviation 1.49 11.59 26.4%2 2.07 12.71 33.46 Standard deviation 1.49 11.59 26.4%2 2.07 12.71 33.46 CV (%) 4.4% 5.2% 6.4% 5.7%% 5.7% 7.8% CV (%) 4.4% 5.2% 6.4% 5.7%% 5.7% 7.8%

Sensitivity

<1 pg/mL

Function

In response to the presence of allergens, this protein ly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10893 MW

Specificity

Natural and recombinant mouse Eotaxin

Anticoagulant

Citrate or EDTA

Gene Name Synonym

Eotaxin

Subcellular Location

Secreted.

Protein Name

Eotaxin

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.048 0.126 0.268 0.526 0.925 1.523 2.298 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.048 0.126 0.268 0.526 0.925 1.523 2.298 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.048 0.126 0.268 0.526 0.925 1.523 2.298 O.D. 0.001 0.048 0.126 0.268 0.526 0.925 1.523 2.298

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.