Mouse EGF ELISA Kit PicoKine®

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 19 20 18 19 0.7 3.6% Sample 2 63 76 72 72 70 4.76 6.8% Sample 3 276 256 275 268 268 7.98 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 19 20 18 19 0.7 3.6% Sample 1 19 19 20 18 19 0.7 3.6% Sample 2 63 76 72 72 70 4.76 6.8% Sample 2 63 76 72 72 70 4.76 6.8% Sample 3 276 256 275 268 268 7.98 2.9% Sample 3 276 256 275 268 268 7.98 2.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 63 276 19 64 270 Standard deviation 1.42 4.34 17.38 1.53 5.44 18.63 CV (%) 7.5% 6.9% 8.5%% 8.1% 8.5% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 63 276 19 64 270 Standard deviation 1.42 4.34 17.38 1.53 5.44 18.63 CV (%) 7.5% 6.9% 8.5%% 8.1% 8.5% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 19 63 276 19 64 270 Mean (pg/ml) 19 63 276 19 64 270 Standard deviation 1.42 4.34 17.38 1.53 5.44 18.63 Standard deviation 1.42 4.34 17.38 1.53 5.44 18.63 CV (%) 7.5% 6.9% 8.5%% 8.1% 8.5% 6.9% CV (%) 7.5% 6.9% 8.5%% 8.1% 8.5% 6.9%

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.03 0.095 0.138 0.229 0.423 0.806 1.391 2.071 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.03 0.095 0.138 0.229 0.423 0.806 1.391 2.071 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.03 0.095 0.138 0.229 0.423 0.806 1.391 2.071 O.D. 0.03 0.095 0.138 0.229 0.423 0.806 1.391 2.071

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.