Human EGF ELISA Kit PicoKine®

Human EGF ELISA Kit PicoKine® (96 Tests) . Quantitate Human EGF in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 11 11 12 11 0.43 3.9% Sample 2 76 76 72 68 73 3.31 4.5% Sample 3 122 118 128 133 125 5.71 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 11 11 12 11 0.43 3.9% Sample 1 11 11 11 12 11 0.43 3.9% Sample 2 76 76 72 68 73 3.31 4.5% Sample 2 76 76 72 68 73 3.31 4.5% Sample 3 122 118 128 133 125 5.71 4.5% Sample 3 122 118 128 133 125 5.71 4.5%

Synonyms

Pro-epidermal growth factor; EGF; Epidermal growth factor; Urogastrone; EGF

Gene Name

EGF

Gene ID

1950

UniProt

E7EVD2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N971-R1023

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in kidney, salivary gland, cerebrum and prostate. .

Applications

ELISA

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Growth Factors, Growth Factors/Hormones, Signal Transduction

Standard

1 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

4.7 pg/mL - 300 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 76 122 10 81 131 Standard deviation 0.71 4.25 9.15 0.82 5.42 12.31 CV (%) 6.5% 5.6% 7.5% 8.2% 6.7% 9.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 76 122 10 81 131 Standard deviation 0.71 4.25 9.15 0.82 5.42 12.31 CV (%) 6.5% 5.6% 7.5% 8.2% 6.7% 9.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 76 122 10 81 131 Mean (pg/ml) 11 76 122 10 81 131 Standard deviation 0.71 4.25 9.15 0.82 5.42 12.31 Standard deviation 0.71 4.25 9.15 0.82 5.42 12.31 CV (%) 6.5% 5.6% 7.5% 8.2% 6.7% 9.4% CV (%) 6.5% 5.6% 7.5% 8.2% 6.7% 9.4%

Sensitivity

<1 pg/mL

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

133994 MW

Specificity

Natural and recombinant human EGF

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Pro-epidermal growth factor

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 9 EGF-like domains.

Protein Name

Pro-epidermal growth factor

Sample Data

Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.007 0.046 0.098 0.192 0.415 0.76 1.502 2.004 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.007 0.046 0.098 0.192 0.415 0.76 1.502 2.004 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.007 0.046 0.098 0.192 0.415 0.76 1.502 2.004 O.D. 0.007 0.046 0.098 0.192 0.415 0.76 1.502 2.004

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.