Rat CNTF ELISA Kit PicoKine®

Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 75 85 81 80 3.63 4.5% Sample 2 276 262 311 268 279 18.99 6.8% Sample 3 868 1021 877 993 939 68.04 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 75 85 81 80 3.63 4.5% Sample 1 82 75 85 81 80 3.63 4.5% Sample 2 276 262 311 268 279 18.99 6.8% Sample 2 276 262 311 268 279 18.99 6.8% Sample 3 868 1021 877 993 939 68.04 7.2% Sample 3 868 1021 877 993 939 68.04 7.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 276 868 80 293 809 Standard deviation 4.59 16.83 60.76 5.52 23.14 60.67 CV (%) 6.1%% 6.1% 7% 6.9% 7.9% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 276 868 80 293 809 Standard deviation 4.59 16.83 60.76 5.52 23.14 60.67 CV (%) 6.1%% 6.1% 7% 6.9% 7.9% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 82 276 868 80 293 809 Mean (pg/ml) 82 276 868 80 293 809 Standard deviation 4.59 16.83 60.76 5.52 23.14 60.67 Standard deviation 4.59 16.83 60.76 5.52 23.14 60.67 CV (%) 6.1%% 6.1% 7% 6.9% 7.9% 7.5% CV (%) 6.1%% 6.1% 7% 6.9% 7.9% 7.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.063 0.126 0.238 0.442 0.838 1.464 2.035 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.063 0.126 0.238 0.442 0.838 1.464 2.035 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.063 0.126 0.238 0.442 0.838 1.464 2.035 O.D. 0.002 0.063 0.126 0.238 0.442 0.838 1.464 2.035

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.