Human BMP-5 ELISA Kit PicoKine®

Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 228 246 239 238 6.68 2.8% Sample 2 1573 1794 1863 1590 1705 126.02 7.3% Sample 3 4363 4914 4157 4495 4482 276.84 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 228 246 239 238 6.68 2.8% Sample 1 242 228 246 239 238 6.68 2.8% Sample 2 1573 1794 1863 1590 1705 126.02 7.3% Sample 2 1573 1794 1863 1590 1705 126.02 7.3% Sample 3 4363 4914 4157 4495 4482 276.84 6.1% Sample 3 4363 4914 4157 4495 4482 276.84 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1573 4363 266 1683 4290 Standard deviation 16.45 69.21 314.13 20.74 82.46 347.49 CV (%) 6.8% 4.4% 7.2% 7.8% 4.9% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1573 4363 266 1683 4290 Standard deviation 16.45 69.21 314.13 20.74 82.46 347.49 CV (%) 6.8% 4.4% 7.2% 7.8% 4.9% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 242 1573 4363 266 1683 4290 Mean (pg/ml) 242 1573 4363 266 1683 4290 Standard deviation 16.45 69.21 314.13 20.74 82.46 347.49 Standard deviation 16.45 69.21 314.13 20.74 82.46 347.49 CV (%) 6.8% 4.4% 7.2% 7.8% 4.9% 8.1% CV (%) 6.8% 4.4% 7.2% 7.8% 4.9% 8.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.064 0.203 0.319 0.534 0.916 1.374 1.946 2.152 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.064 0.203 0.319 0.534 0.916 1.374 1.946 2.152 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.064 0.203 0.319 0.534 0.916 1.374 1.946 2.152 O.D. 0.064 0.203 0.319 0.534 0.916 1.374 1.946 2.152

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.