Human Angiogenin/ANG ELISA Kit PicoKine®

Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 107 121 121 109 114 6.53 5.7% Sample 2 719 697 632 755 700 44.76 6.3% Sample 3 2914 2847 2962 2827 2887 53.74 1.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 107 121 121 109 114 6.53 5.7% Sample 1 107 121 121 109 114 6.53 5.7% Sample 2 719 697 632 755 700 44.76 6.3% Sample 2 719 697 632 755 700 44.76 6.3% Sample 3 2914 2847 2962 2827 2887 53.74 1.8% Sample 3 2914 2847 2962 2827 2887 53.74 1.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 107 719 2914 100 723 2957 Standard deviation 4.81 48.17 195.23 4.6 49.16 251.34 CV (%) 4.5% 6.7% 6.7% 4.6% 6.8% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 107 719 2914 100 723 2957 Standard deviation 4.81 48.17 195.23 4.6 49.16 251.34 CV (%) 4.5% 6.7% 6.7% 4.6% 6.8% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 107 719 2914 100 723 2957 Mean (pg/ml) 107 719 2914 100 723 2957 Standard deviation 4.81 48.17 195.23 4.6 49.16 251.34 Standard deviation 4.81 48.17 195.23 4.6 49.16 251.34 CV (%) 4.5% 6.7% 6.7% 4.6% 6.8% 8.5% CV (%) 4.5% 6.7% 6.7% 4.6% 6.8% 8.5%

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs) . Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.003 0.101 0.143 0.275 0.437 0.889 1.421 2.026 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.003 0.101 0.143 0.275 0.437 0.889 1.421 2.026 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.003 0.101 0.143 0.275 0.437 0.889 1.421 2.026 O.D. 0.003 0.101 0.143 0.275 0.437 0.889 1.421 2.026

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.