Human Amphiregulin (AR) / AREG ELISA Kit PicoKine®

Human Amphiregulin (AR) / AREG ELISA Kit PicoKine® (96 Tests) . Quantitate Human AREG in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha) . This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 27 27 27 0.82 3% Sample 2 200 207 196 193 199 5.24 2.6% Sample 3 441 428 410 450 432 15.03 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 27 27 27 0.82 3% Sample 1 28 29 27 27 27 0.82 3% Sample 2 200 207 196 193 199 5.24 2.6% Sample 2 200 207 196 193 199 5.24 2.6% Sample 3 441 428 410 450 432 15.03 3.4% Sample 3 441 428 410 450 432 15.03 3.4%

Synonyms

Amphiregulin; AR; Colorectum cell-derived growth factor; CRDGF; AREG; SDGF; AREGB

Gene Name

AREG

Gene ID

374

UniProt

P15514

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S101-K198

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Growth Factors, Growth Factors/Hormones, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and human milk.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 200 441 30 197 433 Standard deviation 1.65 10.4 29.54 2.19 10.63 31.6 CV (%) 6.7%% 5.2% 6.7% 7.3% 5.4% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 200 441 30 197 433 Standard deviation 1.65 10.4 29.54 2.19 10.63 31.6 CV (%) 6.7%% 5.2% 6.7% 7.3% 5.4% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 200 441 30 197 433 Mean (pg/ml) 28 200 441 30 197 433 Standard deviation 1.65 10.4 29.54 2.19 10.63 31.6 Standard deviation 1.65 10.4 29.54 2.19 10.63 31.6 CV (%) 6.7%% 5.2% 6.7% 7.3% 5.4% 7.3% CV (%) 6.7%% 5.2% 6.7% 7.3% 5.4% 7.3%

Sensitivity

<10 pg/mL

Function

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27895 MW

Specificity

Natural and recombinant human Amphiregulin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Amphiregulin

Subcellular Location

Membrane; Single-pass membrane protein.

Sequence Similarities

Belongs to the amphiregulin family.

Protein Name

Amphiregulin

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.039 0.063 0.113 0.219 0.451 0.979 1.863 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.039 0.063 0.113 0.219 0.451 0.979 1.863 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.039 0.063 0.113 0.219 0.451 0.979 1.863 O.D. 0.018 0.039 0.063 0.113 0.219 0.451 0.979 1.863

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.