Mouse EGF PicoKine® Quick ELISA Kit

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 19 19 20 19 1.25 6.6% Sample 2 52 63 76 72 66 3.81 5.8% Sample 3 277 256 277 265 269 10.4 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 19 19 20 19 1.25 6.6% Sample 1 18 19 19 20 19 1.25 6.6% Sample 2 52 63 76 72 66 3.81 5.8% Sample 2 52 63 76 72 66 3.81 5.8% Sample 3 277 256 277 265 269 10.4 3.9% Sample 3 277 256 277 265 269 10.4 3.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 52 277 19 63 274 Standard deviation 1.35 3.43 15.24 0.97 5.1 18.08 CV (%) 7.5% 6.6% 5.5% 5.1% 8.1% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 52 277 19 63 274 Standard deviation 1.35 3.43 15.24 0.97 5.1 18.08 CV (%) 7.5% 6.6% 5.5% 5.1% 8.1% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 52 277 19 63 274 Mean (pg/ml) 18 52 277 19 63 274 Standard deviation 1.35 3.43 15.24 0.97 5.1 18.08 Standard deviation 1.35 3.43 15.24 0.97 5.1 18.08 CV (%) 7.5% 6.6% 5.5% 5.1% 8.1% 6.6% CV (%) 7.5% 6.6% 5.5% 5.1% 8.1% 6.6%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.045 0.190 0.322 0.554 0.989 1.746 2.328 2.403 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.045 0.190 0.322 0.554 0.989 1.746 2.328 2.403 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.045 0.190 0.322 0.554 0.989 1.746 2.328 2.403 O.D. 0.045 0.190 0.322 0.554 0.989 1.746 2.328 2.403

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.