Human EGFR PicoKine® Quick ELISA Kit

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.1 It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4) . EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha) . EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 312 285 296 295 14.17 4.8% Sample 2 1679 1695 1722 1688 1696 42.4 2.5% Sample 3 5641 5563 5014 5069 5322 207.55 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 312 285 296 295 14.17 4.8% Sample 1 288 312 285 296 295 14.17 4.8% Sample 2 1679 1695 1722 1688 1696 42.4 2.5% Sample 2 1679 1695 1722 1688 1696 42.4 2.5% Sample 3 5641 5563 5014 5069 5322 207.55 3.9% Sample 3 5641 5563 5014 5069 5322 207.55 3.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1679 5641 324 1765 5711 Standard deviation 13.82 87.31 400.51 20.09 95.31 329.52 CV (%) 4.8% 5.2% 7.1% 6.2% 5.4% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1679 5641 324 1765 5711 Standard deviation 13.82 87.31 400.51 20.09 95.31 329.52 CV (%) 4.8% 5.2% 7.1% 6.2% 5.4% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 288 1679 5641 324 1765 5711 Mean (pg/ml) 288 1679 5641 324 1765 5711 Standard deviation 13.82 87.31 400.51 20.09 95.31 329.52 Standard deviation 13.82 87.31 400.51 20.09 95.31 329.52 CV (%) 4.8% 5.2% 7.1% 6.2% 5.4% 5.8% CV (%) 4.8% 5.2% 7.1% 6.2% 5.4% 5.8%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.010 0.098 0.177 0.314 0.540 0.960 1.572 2.139 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.010 0.098 0.177 0.314 0.540 0.960 1.572 2.139 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.010 0.098 0.177 0.314 0.540 0.960 1.572 2.139 O.D. 0.010 0.098 0.177 0.314 0.540 0.960 1.572 2.139

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.