Rat Endostatin PicoKine® Quick ELISA Kit

Rat Endostatin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Col18a1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 266 249 277 241 258 11.62 4.5% Sample 2 1674 1741 1852 1845 1778 99.57 5.6% Sample 3 4352 4333 4512 4026 4306 167.92 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 266 249 277 241 258 11.62 4.5% Sample 1 266 249 277 241 258 11.62 4.5% Sample 2 1674 1741 1852 1845 1778 99.57 5.6% Sample 2 1674 1741 1852 1845 1778 99.57 5.6% Sample 3 4352 4333 4512 4026 4306 167.92 3.9% Sample 3 4352 4333 4512 4026 4306 167.92 3.9%

Gene Name

Col18a1

Gene ID

85251

UniProt

Q9WUW5

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H1591-K1774

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 266 1674 4352 256 1733 4541 Standard deviation 17.29 102.11 221.95 16.64 93.58 262.02 CV (%) 6.5% 6.1% 5.1% 6.5% 5.4% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 266 1674 4352 256 1733 4541 Standard deviation 17.29 102.11 221.95 16.64 93.58 262.02 CV (%) 6.5% 6.1% 5.1% 6.5% 5.4% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 266 1674 4352 256 1733 4541 Mean (pg/ml) 266 1674 4352 256 1733 4541 Standard deviation 17.29 102.11 221.95 16.64 93.58 262.02 Standard deviation 17.29 102.11 221.95 16.64 93.58 262.02 CV (%) 6.5% 6.1% 5.1% 6.5% 5.4% 5.8% CV (%) 6.5% 6.1% 5.1% 6.5% 5.4% 5.8%

Sensitivity

<15 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant rat Col18a1

Sample Data

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.002 0.109 0.186 0.368 0.747 1.290 1.917 2.267 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.002 0.109 0.186 0.368 0.747 1.290 1.917 2.267 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.002 0.109 0.186 0.368 0.747 1.290 1.917 2.267 O.D. 0.002 0.109 0.186 0.368 0.747 1.290 1.917 2.267

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.