Human BMP-9 PicoKine® Quick ELISA Kit

Human BMP-9 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human GDF2 in bone tissue, cell culture supernatants, serum plasma (heparin, EDTA) and urine. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP) -9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 35 31 32 33 1.97 6% Sample 2 169 181 184 174 177 9.74 5.5% Sample 3 422 405 396 388 403 14.5 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 35 31 32 33 1.97 6% Sample 1 33 35 31 32 33 1.97 6% Sample 2 169 181 184 174 177 9.74 5.5% Sample 2 169 181 184 174 177 9.74 5.5% Sample 3 422 405 396 388 403 14.5 3.6% Sample 3 422 405 396 388 403 14.5 3.6%

Gene Name

GDF2

Gene ID

2658

UniProt

Q9UK05

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: S320-R429

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Bone tissue, cell culture supernatants, serum plasma (heparin, EDTA) and urine

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 169 422 36 174 395 Standard deviation 1.49 8.45 24.48 2.56 12.01 21.73 CV (%) 4.5% 5% 5.8% 7.1% 6.9% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 169 422 36 174 395 Standard deviation 1.49 8.45 24.48 2.56 12.01 21.73 CV (%) 4.5% 5% 5.8% 7.1% 6.9% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 169 422 36 174 395 Mean (pg/ml) 33 169 422 36 174 395 Standard deviation 1.49 8.45 24.48 2.56 12.01 21.73 Standard deviation 1.49 8.45 24.48 2.56 12.01 21.73 CV (%) 4.5% 5% 5.8% 7.1% 6.9% 5.5% CV (%) 4.5% 5% 5.8% 7.1% 6.9% 5.5%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant human GDF2

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.034 0.092 0.137 0.241 0.459 0.796 1.464 2.213 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.034 0.092 0.137 0.241 0.459 0.796 1.464 2.213 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.034 0.092 0.137 0.241 0.459 0.796 1.464 2.213 O.D. 0.034 0.092 0.137 0.241 0.459 0.796 1.464 2.213

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.