Human BMP-9 PicoKine® Quick ELISA Kit

Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP) -9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 35 31 32 33 1.97 6% Sample 2 169 181 184 174 177 9.74 5.5% Sample 3 422 405 396 388 403 14.5 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 35 31 32 33 1.97 6% Sample 1 33 35 31 32 33 1.97 6% Sample 2 169 181 184 174 177 9.74 5.5% Sample 2 169 181 184 174 177 9.74 5.5% Sample 3 422 405 396 388 403 14.5 3.6% Sample 3 422 405 396 388 403 14.5 3.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 169 422 36 174 395 Standard deviation 1.49 8.45 24.48 2.56 12.01 21.73 CV (%) 4.5% 5% 5.8% 7.1% 6.9% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 169 422 36 174 395 Standard deviation 1.49 8.45 24.48 2.56 12.01 21.73 CV (%) 4.5% 5% 5.8% 7.1% 6.9% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 169 422 36 174 395 Mean (pg/ml) 33 169 422 36 174 395 Standard deviation 1.49 8.45 24.48 2.56 12.01 21.73 Standard deviation 1.49 8.45 24.48 2.56 12.01 21.73 CV (%) 4.5% 5% 5.8% 7.1% 6.9% 5.5% CV (%) 4.5% 5% 5.8% 7.1% 6.9% 5.5%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.034 0.092 0.137 0.241 0.459 0.796 1.464 2.213 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.034 0.092 0.137 0.241 0.459 0.796 1.464 2.213 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.034 0.092 0.137 0.241 0.459 0.796 1.464 2.213 O.D. 0.034 0.092 0.137 0.241 0.459 0.796 1.464 2.213

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.