Rat MCP-3/CCL7 PicoKine® Quick ELISA Kit

Rat MCP-3/CCL7 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Ccl7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3) . It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 36 41 40 38 1.69 4.5% Sample 2 236 232 225 230 231 9 3.9% Sample 3 422 471 430 446 442 19.02 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 36 41 40 38 1.69 4.5% Sample 1 33 36 41 40 38 1.69 4.5% Sample 2 236 232 225 230 231 9 3.9% Sample 2 236 232 225 230 231 9 3.9% Sample 3 422 471 430 446 442 19.02 4.3% Sample 3 422 471 430 446 442 19.02 4.3%

Gene Name

CCL7

Gene ID

287561

UniProt

Q9QXY8

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-P97

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 236 422 36 240 452 Standard deviation 2.01 10.38 27.43 1.8 10.56 25.76 CV (%) 6.1% 4.4% 6.5% 5% 4.4% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 236 422 36 240 452 Standard deviation 2.01 10.38 27.43 1.8 10.56 25.76 CV (%) 6.1% 4.4% 6.5% 5% 4.4% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 236 422 36 240 452 Mean (pg/ml) 33 236 422 36 240 452 Standard deviation 2.01 10.38 27.43 1.8 10.56 25.76 Standard deviation 2.01 10.38 27.43 1.8 10.56 25.76 CV (%) 6.1% 4.4% 6.5% 5% 4.4% 5.7% CV (%) 6.1% 4.4% 6.5% 5% 4.4% 5.7%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant rat Ccl7

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.171 0.307 0.552 0.946 1.611 2.060 2.226 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.171 0.307 0.552 0.946 1.611 2.060 2.226 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.171 0.307 0.552 0.946 1.611 2.060 2.226 O.D. 0.025 0.171 0.307 0.552 0.946 1.611 2.060 2.226

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.