Human Kallikrein 5/KLK5 PicoKine® Quick ELISA Kit

Human Kallikrein 5/KLK5 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human KLK5 in cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 139 133 145 153 142 7.38 5.2% Sample 2 814 803 781 813 802 13.63 1.7% Sample 3 2166 2633 2239 2202 2310 189.42 8.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 139 133 145 153 142 7.38 5.2% Sample 1 139 133 145 153 142 7.38 5.2% Sample 2 814 803 781 813 802 13.63 1.7% Sample 2 814 803 781 813 802 13.63 1.7% Sample 3 2166 2633 2239 2202 2310 189.42 8.2% Sample 3 2166 2633 2239 2202 2310 189.42 8.2%

Gene Name

KLK5

Gene ID

25818

UniProt

Q9Y337

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V23-S293

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 139 814 2166 151 752 2373 Standard deviation 5.69 35.81 121.29 9.96 38.35 151.87 CV (%) 4.1% 4.4% 5.6% 6.6% 5.1% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 139 814 2166 151 752 2373 Standard deviation 5.69 35.81 121.29 9.96 38.35 151.87 CV (%) 4.1% 4.4% 5.6% 6.6% 5.1% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 139 814 2166 151 752 2373 Mean (pg/ml) 139 814 2166 151 752 2373 Standard deviation 5.69 35.81 121.29 9.96 38.35 151.87 Standard deviation 5.69 35.81 121.29 9.96 38.35 151.87 CV (%) 4.1% 4.4% 5.6% 6.6% 5.1% 6.4% CV (%) 4.1% 4.4% 5.6% 6.6% 5.1% 6.4%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant human KLK5

Protein Name

Kallikrein-5

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.033 0.096 0.150 0.312 0.430 0.798 1.315 1.913 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.033 0.096 0.150 0.312 0.430 0.798 1.315 1.913 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.033 0.096 0.150 0.312 0.430 0.798 1.315 1.913 O.D. 0.033 0.096 0.150 0.312 0.430 0.798 1.315 1.913

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.