Human Tetranectin/CLEC3B PicoKine® Quick ELISA Kit

Human Tetranectin/CLEC3B PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CLEC3B in cell culture supernatants and serum. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and noncovalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 432 486 505 451 468 29.01 6.2% Sample 2 2688 2756 2929 3088 2865 163.30 5.7% Sample 3 8213 9092 8519 7750 8393 436.43 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 432 486 505 451 468 29.01 6.2% Sample 1 432 486 505 451 468 29.01 6.2% Sample 2 2688 2756 2929 3088 2865 163.30 5.7% Sample 2 2688 2756 2929 3088 2865 163.30 5.7% Sample 3 8213 9092 8519 7750 8393 436.43 5.2% Sample 3 8213 9092 8519 7750 8393 436.43 5.2%

Gene Name

CLEC3B

Gene ID

7123

UniProt

P05452

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-V202

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants and serum

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 432 2688 8213 410 2552 7809 Standard deviation 26.35 118.27 459.92 31.16 137.80 554.43 CV (%) 6.1% 4.4% 5.6% 7.6% 5.4% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 432 2688 8213 410 2552 7809 Standard deviation 26.35 118.27 459.92 31.16 137.80 554.43 CV (%) 6.1% 4.4% 5.6% 7.6% 5.4% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 432 2688 8213 410 2552 7809 Mean (pg/ml) 432 2688 8213 410 2552 7809 Standard deviation 26.35 118.27 459.92 31.16 137.80 554.43 Standard deviation 26.35 118.27 459.92 31.16 137.80 554.43 CV (%) 6.1% 4.4% 5.6% 7.6% 5.4% 7.1% CV (%) 6.1% 4.4% 5.6% 7.6% 5.4% 7.1%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant human CLEC3B

Sample Data

Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.002 0.123 0.215 0.377 0.703 1.153 1.707 2.189 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.002 0.123 0.215 0.377 0.703 1.153 1.707 2.189 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.002 0.123 0.215 0.377 0.703 1.153 1.707 2.189 O.D. 0.002 0.123 0.215 0.377 0.703 1.153 1.707 2.189

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.