Human CCL15/Mip 1 Delta PicoKine® Quick ELISA Kit

Human CCL15/Mip 1 Delta PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CCL15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 15 (CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 34 34 38 36 2.26 6.3% Sample 2 190 190 186 189 188 3.19 1.7% Sample 3 444 435 482 452 453 23.55 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 34 34 38 36 2.26 6.3% Sample 1 38 34 34 38 36 2.26 6.3% Sample 2 190 190 186 189 188 3.19 1.7% Sample 2 190 190 186 189 188 3.19 1.7% Sample 3 444 435 482 452 453 23.55 5.2% Sample 3 444 435 482 452 453 23.55 5.2%

Gene Name

CCL15

Gene ID

6359

UniProt

Q16663

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q22-I113

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 190 444 36 192 422 Standard deviation 2.69 10.26 33.74 3.09 16.12 38.40 CV (%) 7.1% 5.4% 7.6% 8.6% 8.4% 9.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 190 444 36 192 422 Standard deviation 2.69 10.26 33.74 3.09 16.12 38.40 CV (%) 7.1% 5.4% 7.6% 8.6% 8.4% 9.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 38 190 444 36 192 422 Mean (pg/ml) 38 190 444 36 192 422 Standard deviation 2.69 10.26 33.74 3.09 16.12 38.40 Standard deviation 2.69 10.26 33.74 3.09 16.12 38.40 CV (%) 7.1% 5.4% 7.6% 8.6% 8.4% 9.1% CV (%) 7.1% 5.4% 7.6% 8.6% 8.4% 9.1%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant human CCL15

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.026 0.101 0.165 0.287 0.524 0.951 1.569 2.145 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.026 0.101 0.165 0.287 0.524 0.951 1.569 2.145 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.026 0.101 0.165 0.287 0.524 0.951 1.569 2.145 O.D. 0.026 0.101 0.165 0.287 0.524 0.951 1.569 2.145

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.