Human CCL14/HCC-1 PicoKine® Quick ELISA Kit

Human CCL14/HCC-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CCL14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 42 39 41 40 40 1.36 3.4% Sample 2 232 255 256 221 241 13.49 5.6% Sample 3 450 506 528 504 497 25.84 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 42 39 41 40 40 1.36 3.4% Sample 1 42 39 41 40 40 1.36 3.4% Sample 2 232 255 256 221 241 13.49 5.6% Sample 2 232 255 256 221 241 13.49 5.6% Sample 3 450 506 528 504 497 25.84 5.2% Sample 3 450 506 528 504 497 25.84 5.2%

Gene Name

CCL14

Gene ID

6358

UniProt

Q16627

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T20-N93

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 232 450 45 232 451 Standard deviation 2.14 12.99 25.20 2.97 17.16 34.72 CV (%) 5.1% 5.6% 5.6% 6.6% 7.4% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 232 450 45 232 451 Standard deviation 2.14 12.99 25.20 2.97 17.16 34.72 CV (%) 5.1% 5.6% 5.6% 6.6% 7.4% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 42 232 450 45 232 451 Mean (pg/ml) 42 232 450 45 232 451 Standard deviation 2.14 12.99 25.20 2.97 17.16 34.72 Standard deviation 2.14 12.99 25.20 2.97 17.16 34.72 CV (%) 5.1% 5.6% 5.6% 6.6% 7.4% 7.7% CV (%) 5.1% 5.6% 5.6% 6.6% 7.4% 7.7%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant human CCL14

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.023 0.092 0.163 0.208 0.563 1.085 1.851 2.381 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.023 0.092 0.163 0.208 0.563 1.085 1.851 2.381 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.023 0.092 0.163 0.208 0.563 1.085 1.851 2.381 O.D. 0.023 0.092 0.163 0.208 0.563 1.085 1.851 2.381

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.