Mouse CCL17/TARC PicoKine® Quick ELISA Kit

Mouse CCL17/TARC PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Ccl17 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC) . CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 The standard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 64 61 61 65 62 2.17 3.5% Sample 2 214 195 236 212 214 14.12 6.6% Sample 3 1001 968 972 956 974 21.42 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 64 61 61 65 62 2.17 3.5% Sample 1 64 61 61 65 62 2.17 3.5% Sample 2 214 195 236 212 214 14.12 6.6% Sample 2 214 195 236 212 214 14.12 6.6% Sample 3 1001 968 972 956 974 21.42 2.2% Sample 3 1001 968 972 956 974 21.42 2.2%

Gene Name

Ccl17

Gene ID

20295

UniProt

F6R5P4

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-P93

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 214 1001 60 231 1081 Standard deviation 3.26 11.77 62.06 3.96 17.09 83.23 CV (%) 5.1% 5.5% 6.2% 6.6% 7.4% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 214 1001 60 231 1081 Standard deviation 3.26 11.77 62.06 3.96 17.09 83.23 CV (%) 5.1% 5.5% 6.2% 6.6% 7.4% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 64 214 1001 60 231 1081 Mean (pg/ml) 64 214 1001 60 231 1081 Standard deviation 3.26 11.77 62.06 3.96 17.09 83.23 Standard deviation 3.26 11.77 62.06 3.96 17.09 83.23 CV (%) 5.1% 5.5% 6.2% 6.6% 7.4% 7.7% CV (%) 5.1% 5.5% 6.2% 6.6% 7.4% 7.7%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant mouse Ccl17

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.051 0.135 0.269 0.447 0.761 1.298 1.998 2.321 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.051 0.135 0.269 0.447 0.761 1.298 1.998 2.321 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.051 0.135 0.269 0.447 0.761 1.298 1.998 2.321 O.D. 0.051 0.135 0.269 0.447 0.761 1.298 1.998 2.321

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.