Human CCL17/TARC PicoKine® Quick ELISA Kit

Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC) . CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 The standard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 67 63 65 59 63 2.20 3.5% Sample 2 265 292 252 282 272 18.49 6.8% Sample 3 948 991 998 991 982 20.62 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 67 63 65 59 63 2.20 3.5% Sample 1 67 63 65 59 63 2.20 3.5% Sample 2 265 292 252 282 272 18.49 6.8% Sample 2 265 292 252 282 272 18.49 6.8% Sample 3 948 991 998 991 982 20.62 2.1% Sample 3 948 991 998 991 982 20.62 2.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 67 265 948 61 241 936 Standard deviation 2.94 11.92 49.29 3.05 13.49 62.71 CV (%) 4.4% 4.5% 5.2% 5.0% 5.6% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 67 265 948 61 241 936 Standard deviation 2.94 11.92 49.29 3.05 13.49 62.71 CV (%) 4.4% 4.5% 5.2% 5.0% 5.6% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 67 265 948 61 241 936 Mean (pg/ml) 67 265 948 61 241 936 Standard deviation 2.94 11.92 49.29 3.05 13.49 62.71 Standard deviation 2.94 11.92 49.29 3.05 13.49 62.71 CV (%) 4.4% 4.5% 5.2% 5.0% 5.6% 6.7% CV (%) 4.4% 4.5% 5.2% 5.0% 5.6% 6.7%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.031 0.169 0.281 0.498 0.905 1.322 1.583 1.912 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.031 0.169 0.281 0.498 0.905 1.322 1.583 1.912 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.031 0.169 0.281 0.498 0.905 1.322 1.583 1.912 O.D. 0.031 0.169 0.281 0.498 0.905 1.322 1.583 1.912

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.