Human CD55 PicoKine® Quick ELISA Kit

Human CD55 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CD55 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. It is mapped to 1q32.2. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM) . Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 552 524 499 503 520 35.85 6.9% Sample 2 2036 2144 2161 2019 2103 46.26 2.2% Sample 3 8896 8941 9041 9569 9112 309.8 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 552 524 499 503 520 35.85 6.9% Sample 1 552 524 499 503 520 35.85 6.9% Sample 2 2036 2144 2161 2019 2103 46.26 2.2% Sample 2 2036 2144 2161 2019 2103 46.26 2.2% Sample 3 8896 8941 9041 9569 9112 309.8 3.4% Sample 3 8896 8941 9041 9569 9112 309.8 3.4%

Gene Name

CD55

Gene ID

1604

UniProt

P08174

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D35-S353

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 552 2036 8896 584 2230 9523 Standard deviation 34.78 126.23 498.18 35.62 102.58 657.09 CV (%) 6.3% 6.2% 5.6% 6.1% 4.6% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 552 2036 8896 584 2230 9523 Standard deviation 34.78 126.23 498.18 35.62 102.58 657.09 CV (%) 6.3% 6.2% 5.6% 6.1% 4.6% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 552 2036 8896 584 2230 9523 Mean (pg/ml) 552 2036 8896 584 2230 9523 Standard deviation 34.78 126.23 498.18 35.62 102.58 657.09 Standard deviation 34.78 126.23 498.18 35.62 102.58 657.09 CV (%) 6.3% 6.2% 5.6% 6.1% 4.6% 6.9% CV (%) 6.3% 6.2% 5.6% 6.1% 4.6% 6.9%

Sensitivity

<50 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CD55

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.000 0.071 0.156 0.292 0.517 0.919 1.468 2.039 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.000 0.071 0.156 0.292 0.517 0.919 1.468 2.039 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.000 0.071 0.156 0.292 0.517 0.919 1.468 2.039 O.D. 0.000 0.071 0.156 0.292 0.517 0.919 1.468 2.039

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.