Human KIT/SCFR/CD117/C Kit PicoKine® Quick ELISA Kit

Human KIT/SCFR/CD117/C Kit PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human KIT/SCFR/CD117/C Kit in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

SCFR (Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 342 325 296 336 325 15.91 4.9% Sample 2 1263 1226 1254 1196 1235 39.51 3.2% Sample 3 4698 4475 4562 4369 4526 190.09 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 342 325 296 336 325 15.91 4.9% Sample 1 342 325 296 336 325 15.91 4.9% Sample 2 1263 1226 1254 1196 1235 39.51 3.2% Sample 2 1263 1226 1254 1196 1235 39.51 3.2% Sample 3 4698 4475 4562 4369 4526 190.09 4.2% Sample 3 4698 4475 4562 4369 4526 190.09 4.2%

Gene Name

KIT

Gene ID

3815

UniProt

P10721

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q26-T520

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 342 1263 4698 351 1236 4352 Standard deviation 20.18 54.31 272.48 24.92 69.22 278.53 CV (%) 5.9% 4.3% 5.8% 7.1% 5.6% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 342 1263 4698 351 1236 4352 Standard deviation 20.18 54.31 272.48 24.92 69.22 278.53 CV (%) 5.9% 4.3% 5.8% 7.1% 5.6% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 342 1263 4698 351 1236 4352 Mean (pg/ml) 342 1263 4698 351 1236 4352 Standard deviation 20.18 54.31 272.48 24.92 69.22 278.53 Standard deviation 20.18 54.31 272.48 24.92 69.22 278.53 CV (%) 5.9% 4.3% 5.8% 7.1% 5.6% 6.4% CV (%) 5.9% 4.3% 5.8% 7.1% 5.6% 6.4%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human KIT

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.078 0.177 0.341 0.650 0.930 1.389 1.645 2.068 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.078 0.177 0.341 0.650 0.930 1.389 1.645 2.068 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.078 0.177 0.341 0.650 0.930 1.389 1.645 2.068 O.D. 0.078 0.177 0.341 0.650 0.930 1.389 1.645 2.068

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.