Mouse CD44 PicoKine® Quick ELISA Kit

CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 778 783 658 694 728 31.31 4.3% Sample 2 1941 1958 1941 2136 1994 69.79 3.5% Sample 3 8310 7950 8440 8644 8336 233.42 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 778 783 658 694 728 31.31 4.3% Sample 1 778 783 658 694 728 31.31 4.3% Sample 2 1941 1958 1941 2136 1994 69.79 3.5% Sample 2 1941 1958 1941 2136 1994 69.79 3.5% Sample 3 8310 7950 8440 8644 8336 233.42 2.8% Sample 3 8310 7950 8440 8644 8336 233.42 2.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 778 1941 8310 763 1853 7959 Standard deviation 43.57 137.81 623.25 52.65 120.45 541.21 CV (%) 5.6% 7.1% 7.5% 6.9% 6.5% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 778 1941 8310 763 1853 7959 Standard deviation 43.57 137.81 623.25 52.65 120.45 541.21 CV (%) 5.6% 7.1% 7.5% 6.9% 6.5% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 778 1941 8310 763 1853 7959 Mean (pg/ml) 778 1941 8310 763 1853 7959 Standard deviation 43.57 137.81 623.25 52.65 120.45 541.21 Standard deviation 43.57 137.81 623.25 52.65 120.45 541.21 CV (%) 5.6% 7.1% 7.5% 6.9% 6.5% 6.8% CV (%) 5.6% 7.1% 7.5% 6.9% 6.5% 6.8%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.029 0.071 0.162 0.277 0.471 0.868 1.470 2.161 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.029 0.071 0.162 0.277 0.471 0.868 1.470 2.161 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.029 0.071 0.162 0.277 0.471 0.868 1.470 2.161 O.D. 0.029 0.071 0.162 0.277 0.471 0.868 1.470 2.161

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.