Human IL-34/Interleukin-34 PicoKine® Quick ELISA Kit

Human IL-34/Interleukin-34 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human IL-34/Interleukin-34 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It is mapped to 16q22.1. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 is a recently discovered cytokine that acts on tissue resident macrophages and Langerhans cells upon binding the receptor for CSF-1, CSF-1R. It also can promote the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor. In addition, it promotes the development, survival, and function of microglia and Langerhans cells, therefore, this cytokine may predominately function in brain and skin biology.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 88 90 89 91 4.07 4.5% Sample 2 541 522 541 496 525 17.85 3.4% Sample 3 1211 1362 1474 1496 1386 81.76 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 88 90 89 91 4.07 4.5% Sample 1 95 88 90 89 91 4.07 4.5% Sample 2 541 522 541 496 525 17.85 3.4% Sample 2 541 522 541 496 525 17.85 3.4% Sample 3 1211 1362 1474 1496 1386 81.76 5.9% Sample 3 1211 1362 1474 1496 1386 81.76 5.9%

Gene Name

IL34

Gene ID

146433

UniProt

Q6ZMJ4

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N21-P242

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 141 1211 97 521 1165 Standard deviation 4.28 35.71 71.45 5.63 32.82 71.07 CV (%) 4.5% 6.6% 5.9% 5.8% 6.3% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 141 1211 97 521 1165 Standard deviation 4.28 35.71 71.45 5.63 32.82 71.07 CV (%) 4.5% 6.6% 5.9% 5.8% 6.3% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 95 141 1211 97 521 1165 Mean (pg/ml) 95 141 1211 97 521 1165 Standard deviation 4.28 35.71 71.45 5.63 32.82 71.07 Standard deviation 4.28 35.71 71.45 5.63 32.82 71.07 CV (%) 4.5% 6.6% 5.9% 5.8% 6.3% 6.1% CV (%) 4.5% 6.6% 5.9% 5.8% 6.3% 6.1%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human IL34

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 1.761 2.064 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 1.761 2.064 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 1.761 2.064 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 1.761 2.064

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.