Human CD40/TNFRSF5 PicoKine® Quick ELISA Kit

Human CD40/TNFRSF5 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CD40/TNFRSF5 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 31 34 35 33 2.19 6.6% Sample 2 165 170 178 180 173 7.8 4.5% Sample 3 415 425 433 501 444 11.53 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 31 34 35 33 2.19 6.6% Sample 1 33 31 34 35 33 2.19 6.6% Sample 2 165 170 178 180 173 7.8 4.5% Sample 2 165 170 178 180 173 7.8 4.5% Sample 3 415 425 433 501 444 11.53 2.6% Sample 3 415 425 433 501 444 11.53 2.6%

Gene Name

CD40

Gene ID

958

UniProt

P25942

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-R193

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 165 415 34 181 409 Standard deviation 1.49 7.92 16.19 2.01 11.22 35.99 CV (%) 4.5% 4.8% 3.9% 5.9% 6.2% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 165 415 34 181 409 Standard deviation 1.49 7.92 16.19 2.01 11.22 35.99 CV (%) 4.5% 4.8% 3.9% 5.9% 6.2% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 165 415 34 181 409 Mean (pg/ml) 33 165 415 34 181 409 Standard deviation 1.49 7.92 16.19 2.01 11.22 35.99 Standard deviation 1.49 7.92 16.19 2.01 11.22 35.99 CV (%) 4.5% 4.8% 3.9% 5.9% 6.2% 8.8% CV (%) 4.5% 4.8% 3.9% 5.9% 6.2% 8.8%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CD40

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.031 0.114 0.180 0.315 0.559 0.991 1.694 2.208 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.031 0.114 0.180 0.315 0.559 0.991 1.694 2.208 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.031 0.114 0.180 0.315 0.559 0.991 1.694 2.208 O.D. 0.031 0.114 0.180 0.315 0.559 0.991 1.694 2.208

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.