Mouse CCL12/MCP5 PicoKine® Quick ELISA Kit

Mouse CCL12/MCP5 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse CCL12/MCP51 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 12 (CCL12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5 (MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominately in lymph nodes and thymus under normal conditions, and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens. The gene for CCL12 is found in a cluster of CC chemokines on mouse chromosome 11.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 90 78 82 84 4.28 5.1% Sample 2 251 284 278 261 268 13.13 4.9% Sample 3 901 899 850 891 885 45.13 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 90 78 82 84 4.28 5.1% Sample 1 86 90 78 82 84 4.28 5.1% Sample 2 251 284 278 261 268 13.13 4.9% Sample 2 251 284 278 261 268 13.13 4.9% Sample 3 901 899 850 891 885 45.13 5.1% Sample 3 901 899 850 891 885 45.13 5.1%

Gene Name

Ccl12

Gene ID

20293

UniProt

Q62401

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G23-G104

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 241 901 86 245 988 Standard deviation 5.59 15.67 40.55 7.04 15.44 60.27 CV (%) 6.5% 6.5% 4.5% 8% 6.3% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 241 901 86 245 988 Standard deviation 5.59 15.67 40.55 7.04 15.44 60.27 CV (%) 6.5% 6.5% 4.5% 8% 6.3% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 86 241 901 86 245 988 Mean (pg/ml) 86 241 901 86 245 988 Standard deviation 5.59 15.67 40.55 7.04 15.44 60.27 Standard deviation 5.59 15.67 40.55 7.04 15.44 60.27 CV (%) 6.5% 6.5% 4.5% 8% 6.3% 6.1% CV (%) 6.5% 6.5% 4.5% 8% 6.3% 6.1%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Ccl12

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.048 0.081 0.153 0.283 0.485 0.866 1.482 2.155 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.048 0.081 0.153 0.283 0.485 0.866 1.482 2.155 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.048 0.081 0.153 0.283 0.485 0.866 1.482 2.155 O.D. 0.048 0.081 0.153 0.283 0.485 0.866 1.482 2.155

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.