Human PECAM-1/CD31 PicoKine® Quick ELISA Kit

Human PECAM-1/CD31 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human PECAM-1/CD31 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Human platelet/endothelial cell adhesion molecule-1 (PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to be understood, but its function in vivo is yet to be established.1 The PECAM1 locus is on the long arm of chromosome 17, in the region q23-qter. To confirm this observation and obtain a more precise localization of the PECAM1 locus, fluorescence in situ hybridization was conducted. Together our data allowed assignment of the PECAM1 locus to the region 17q23.2 To examine the functional role of PECAM-1 in regulating platelet-collagen interactions, 2 different approaches were applied using recombinant human PECAM-1-immunoglobulin chimeras and platelets derived from PECAM-1-deficient mice.3 PECAM-1, an integral membrane protein with an essential role in TEM, is found in this compartment and constitutively recycles evenly along endothelial cell borders.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 574 amino acids (Q28-K601) with the molecular weight of 64.5KDa. After glycosylating, its molecular weight changes to 90-100KDa.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 294 274 310 279 289 17.07 5.9% Sample 2 1324 1342 1541 1368 1394 71.08 5.1% Sample 3 4881 4242 4615 4269 4502 216.08 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 294 274 310 279 289 17.07 5.9% Sample 1 294 274 310 279 289 17.07 5.9% Sample 2 1324 1342 1541 1368 1394 71.08 5.1% Sample 2 1324 1342 1541 1368 1394 71.08 5.1% Sample 3 4881 4242 4615 4269 4502 216.08 4.8% Sample 3 4881 4242 4615 4269 4502 216.08 4.8%

Gene Name

PECAM1

Gene ID

5175

UniProt

P16284

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q28-K601

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 294 1324 4881 296 1456 4803 Standard deviation 11.17 88.71 312.38 16.87 115.02 307.39 CV (%) 3.8% 6.7% 6.4% 5.7% 7.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 294 1324 4881 296 1456 4803 Standard deviation 11.17 88.71 312.38 16.87 115.02 307.39 CV (%) 3.8% 6.7% 6.4% 5.7% 7.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 294 1324 4881 296 1456 4803 Mean (pg/ml) 294 1324 4881 296 1456 4803 Standard deviation 11.17 88.71 312.38 16.87 115.02 307.39 Standard deviation 11.17 88.71 312.38 16.87 115.02 307.39 CV (%) 3.8% 6.7% 6.4% 5.7% 7.9% 6.4% CV (%) 3.8% 6.7% 6.4% 5.7% 7.9% 6.4%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human PECAM1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.004 0.121 0.235 0.435 0.778 1.316 1.880 2.269 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.004 0.121 0.235 0.435 0.778 1.316 1.880 2.269 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.004 0.121 0.235 0.435 0.778 1.316 1.880 2.269 O.D. 0.004 0.121 0.235 0.435 0.778 1.316 1.880 2.269

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.