Mouse TNFRSF7/CD27 PicoKine® Quick ELISA Kit

CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 85 81 93 86 3.77 4.4% Sample 2 579 605 594 616 599 31.72 5.3% Sample 3 1861 1745 2051 2133 1948 138.27 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 85 81 93 86 3.77 4.4% Sample 1 84 85 81 93 86 3.77 4.4% Sample 2 579 605 594 616 599 31.72 5.3% Sample 2 579 605 594 616 599 31.72 5.3% Sample 3 1861 1745 2051 2133 1948 138.27 7.1% Sample 3 1861 1745 2051 2133 1948 138.27 7.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 579 1861 79 541 2073 Standard deviation 4.28 44 109.8 3.87 33.54 101.58 CV (%) 5.1% 7.6% 5.9% 4.9% 6.2% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 579 1861 79 541 2073 Standard deviation 4.28 44 109.8 3.87 33.54 101.58 CV (%) 5.1% 7.6% 5.9% 4.9% 6.2% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 84 579 1861 79 541 2073 Mean (pg/ml) 84 579 1861 79 541 2073 Standard deviation 4.28 44 109.8 3.87 33.54 101.58 Standard deviation 4.28 44 109.8 3.87 33.54 101.58 CV (%) 5.1% 7.6% 5.9% 4.9% 6.2% 4.9% CV (%) 5.1% 7.6% 5.9% 4.9% 6.2% 4.9%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.052 0.153 0.270 0.448 0.762 1.270 1.870 2.200 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.052 0.153 0.270 0.448 0.762 1.270 1.870 2.200 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.052 0.153 0.270 0.448 0.762 1.270 1.870 2.200 O.D. 0.052 0.153 0.270 0.448 0.762 1.270 1.870 2.200

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.