Mouse CCL25/TECK PicoKine® Quick ELISA Kit

Mouse CCL25/TECK PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse CCL25/TECK in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed Chemokine) . The mouse Teck gene was mapped to chromosome 8 and human Teck gene was to 19p13.2. CCL25 is believed to play a role in the development of T-cells. It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 elicits its effects by binding to the chemokine receptor CCR9.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 98 86 97 93 3.08 3.3% Sample 2 264 284 257 266 268 18.47 6.9% Sample 3 941 985 981 953 965 27.99 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 98 86 97 93 3.08 3.3% Sample 1 92 98 86 97 93 3.08 3.3% Sample 2 264 284 257 266 268 18.47 6.9% Sample 2 264 284 257 266 268 18.47 6.9% Sample 3 941 985 981 953 965 27.99 2.9% Sample 3 941 985 981 953 965 27.99 2.9%

Gene Name

Ccl25

Gene ID

20300

UniProt

O35903

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-N144

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 364 941 97 276 984 Standard deviation 4.97 17.42 70.58 5.92 20.7 66.91 CV (%) 5.4% 6.6% 7.5% 6.1% 7.5% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 364 941 97 276 984 Standard deviation 4.97 17.42 70.58 5.92 20.7 66.91 CV (%) 5.4% 6.6% 7.5% 6.1% 7.5% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 92 364 941 97 276 984 Mean (pg/ml) 92 364 941 97 276 984 Standard deviation 4.97 17.42 70.58 5.92 20.7 66.91 Standard deviation 4.97 17.42 70.58 5.92 20.7 66.91 CV (%) 5.4% 6.6% 7.5% 6.1% 7.5% 6.8% CV (%) 5.4% 6.6% 7.5% 6.1% 7.5% 6.8%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Ccl25

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.