Mouse CCL25/TECK PicoKine® Quick ELISA Kit

Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed Chemokine) . The mouse Teck gene was mapped to chromosome 8 and human Teck gene was to 19p13.2. CCL25 is believed to play a role in the development of T-cells. It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 elicits its effects by binding to the chemokine receptor CCR9.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 98 86 97 93 3.08 3.3% Sample 2 264 284 257 266 268 18.47 6.9% Sample 3 941 985 981 953 965 27.99 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 98 86 97 93 3.08 3.3% Sample 1 92 98 86 97 93 3.08 3.3% Sample 2 264 284 257 266 268 18.47 6.9% Sample 2 264 284 257 266 268 18.47 6.9% Sample 3 941 985 981 953 965 27.99 2.9% Sample 3 941 985 981 953 965 27.99 2.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 364 941 97 276 984 Standard deviation 4.97 17.42 70.58 5.92 20.7 66.91 CV (%) 5.4% 6.6% 7.5% 6.1% 7.5% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 364 941 97 276 984 Standard deviation 4.97 17.42 70.58 5.92 20.7 66.91 CV (%) 5.4% 6.6% 7.5% 6.1% 7.5% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 92 364 941 97 276 984 Mean (pg/ml) 92 364 941 97 276 984 Standard deviation 4.97 17.42 70.58 5.92 20.7 66.91 Standard deviation 4.97 17.42 70.58 5.92 20.7 66.91 CV (%) 5.4% 6.6% 7.5% 6.1% 7.5% 6.8% CV (%) 5.4% 6.6% 7.5% 6.1% 7.5% 6.8%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.