Human CCL13/MCP4 PicoKine® Quick ELISA Kit

Human CCL13/MCP4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CCL13/MCP4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 13 (CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 18 17 17 18 1.26 7.1% Sample 2 87 85 88 82 86 2.05 2.4% Sample 3 258 234 248 251 248 11.15 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 18 17 17 18 1.26 7.1% Sample 1 19 18 17 17 18 1.26 7.1% Sample 2 87 85 88 82 86 2.05 2.4% Sample 2 87 85 88 82 86 2.05 2.4% Sample 3 258 234 248 251 248 11.15 4.5% Sample 3 258 234 248 251 248 11.15 4.5%

Gene Name

CCL13

Gene ID

6357

UniProt

Q99616

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-T98

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

7.8 pg/mL - 500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 87 258 18 85 271 Standard deviation 1.16 5.39 17.8 1.26 5.44 15.91 CV (%) 6.1% 6.2% 6.9% 7% 6.4% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 87 258 18 85 271 Standard deviation 1.16 5.39 17.8 1.26 5.44 15.91 CV (%) 6.1% 6.2% 6.9% 7% 6.4% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 19 87 258 18 85 271 Mean (pg/ml) 19 87 258 18 85 271 Standard deviation 1.16 5.39 17.8 1.26 5.44 15.91 Standard deviation 1.16 5.39 17.8 1.26 5.44 15.91 CV (%) 6.1% 6.2% 6.9% 7% 6.4% 7.2% CV (%) 6.1% 6.2% 6.9% 7% 6.4% 7.2%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CCL13

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.054 0.198 0.325 0.579 0.992 1.672 2.213 2.565 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.054 0.198 0.325 0.579 0.992 1.672 2.213 2.565 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.054 0.198 0.325 0.579 0.992 1.672 2.213 2.565 O.D. 0.054 0.198 0.325 0.579 0.992 1.672 2.213 2.565

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.