Mouse CCL24/Eotaxin-2 PicoKine® Quick ELISA Kit

Mouse CCL24/Eotaxin-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse CCL24/Eotaxin-2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 24 (CCL24) also known as myeloid progenitor inhibitory factor 2 (MPIF-2) or eosinophil chemotactic protein 2 (eotaxin-2) is a protein that in humans is encoded by the CCL24 gene.1 By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23.2 CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 42 45 39 0.85 2.2% Sample 2 230 192 217 197 209 9.41 4.5% Sample 3 491 488 471 469 480 29.26 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 42 45 39 0.85 2.2% Sample 1 33 34 42 45 39 0.85 2.2% Sample 2 230 192 217 197 209 9.41 4.5% Sample 2 230 192 217 197 209 9.41 4.5% Sample 3 491 488 471 469 480 29.26 6.1% Sample 3 491 488 471 469 480 29.26 6.1%

Gene Name

Ccl24

Gene ID

56221

UniProt

Q9JKC0

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V27-V119

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 230 491 42 209 487 Standard deviation 1.95 10.81 33.88 2.86 12.33 38.47 CV (%) 5.9% 4.7% 6.9% 6.8% 5.9% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 230 491 42 209 487 Standard deviation 1.95 10.81 33.88 2.86 12.33 38.47 CV (%) 5.9% 4.7% 6.9% 6.8% 5.9% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 230 491 42 209 487 Mean (pg/ml) 33 230 491 42 209 487 Standard deviation 1.95 10.81 33.88 2.86 12.33 38.47 Standard deviation 1.95 10.81 33.88 2.86 12.33 38.47 CV (%) 5.9% 4.7% 6.9% 6.8% 5.9% 7.9% CV (%) 5.9% 4.7% 6.9% 6.8% 5.9% 7.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Ccl24

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.025 0.121 0.199 0.326 0.638 1.123 1.913 2.483 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.025 0.121 0.199 0.326 0.638 1.123 1.913 2.483 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.025 0.121 0.199 0.326 0.638 1.123 1.913 2.483 O.D. 0.025 0.121 0.199 0.326 0.638 1.123 1.913 2.483

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.