Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit

Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CCL21/6Ckine/Exodus 2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 12 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC) . By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 117 107 102 115 110 6.06 5.5% Sample 2 825 788 952 906 868 15.62 1.8% Sample 3 1412 1542 1469 1397 1455 59.66 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 117 107 102 115 110 6.06 5.5% Sample 1 117 107 102 115 110 6.06 5.5% Sample 2 825 788 952 906 868 15.62 1.8% Sample 2 825 788 952 906 868 15.62 1.8% Sample 3 1412 1542 1469 1397 1455 59.66 4.1% Sample 3 1412 1542 1469 1397 1455 59.66 4.1%

Gene Name

CCL21

Gene ID

6366

UniProt

O00585

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-P134

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 117 825 1412 108 794 1510 Standard deviation 4.56 30.53 83.31 7.02 34.94 92.11 CV (%) 3.9% 3.7% 5.9% 6.5% 4.4% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 117 825 1412 108 794 1510 Standard deviation 4.56 30.53 83.31 7.02 34.94 92.11 CV (%) 3.9% 3.7% 5.9% 6.5% 4.4% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 117 825 1412 108 794 1510 Mean (pg/ml) 117 825 1412 108 794 1510 Standard deviation 4.56 30.53 83.31 7.02 34.94 92.11 Standard deviation 4.56 30.53 83.31 7.02 34.94 92.11 CV (%) 3.9% 3.7% 5.9% 6.5% 4.4% 6.1% CV (%) 3.9% 3.7% 5.9% 6.5% 4.4% 6.1%

Sensitivity

<12 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CCL21

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.010 0.062 0.139 0.313 0.475 0.802 1.584 2.096 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.010 0.062 0.139 0.313 0.475 0.802 1.584 2.096 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.010 0.062 0.139 0.313 0.475 0.802 1.584 2.096 O.D. 0.010 0.062 0.139 0.313 0.475 0.802 1.584 2.096

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.