Human CCL24/Eotaxin-2 PicoKine® Quick ELISA Kit

Chemokine (C-C motif) ligand 24 (CCL24) also known as myeloid progenitor inhibitory factor 2 (MPIF-2) or eosinophil chemotactic protein 2 (eotaxin-2) is a protein that in humans is encoded by the CCL24 gene. By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23. CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 88 76 69 78 3.74 4.8% Sample 2 211 190 202 206 202 7.69 3.8% Sample 3 864 1004 899 921 922 67.31 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 88 76 69 78 3.74 4.8% Sample 1 79 88 76 69 78 3.74 4.8% Sample 2 211 190 202 206 202 7.69 3.8% Sample 2 211 190 202 206 202 7.69 3.8% Sample 3 864 1004 899 921 922 67.31 7.3% Sample 3 864 1004 899 921 922 67.31 7.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 211 864 76 231 787 Standard deviation 3.87 12.87 47.52 3.65 15.94 46.43 CV (%) 4.9% 6.1% 5.5% 4.8% 6.9% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 211 864 76 231 787 Standard deviation 3.87 12.87 47.52 3.65 15.94 46.43 CV (%) 4.9% 6.1% 5.5% 4.8% 6.9% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 79 211 864 76 231 787 Mean (pg/ml) 79 211 864 76 231 787 Standard deviation 3.87 12.87 47.52 3.65 15.94 46.43 Standard deviation 3.87 12.87 47.52 3.65 15.94 46.43 CV (%) 4.9% 6.1% 5.5% 4.8% 6.9% 5.9% CV (%) 4.9% 6.1% 5.5% 4.8% 6.9% 5.9%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.001 0.242 0.448 0.855 1.494 2.270 2.488 2.473 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.001 0.242 0.448 0.855 1.494 2.270 2.488 2.473 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.001 0.242 0.448 0.855 1.494 2.270 2.488 2.473 O.D. 0.001 0.242 0.448 0.855 1.494 2.270 2.488 2.473

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.