Human CES1 PicoKine® Quick ELISA Kit

Human CES1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CES1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 0.1 ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Liver carboxylesterase 1 also known as carboxylesterase 1 (CES1, hCE-1 or CES1A1) is an enzyme that in humans is encoded by the CES1 gene. This gene is mapped to 16q12.2. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1504 1621 1563 1509 1549 47.44 3% Sample 2 6502 6771 6290 6265 6457 203.33 3.1% Sample 3 26206 23293 23327 24983 24452 1221.41 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1504 1621 1563 1509 1549 47.44 3% Sample 1 1504 1621 1563 1509 1549 47.44 3% Sample 2 6502 6771 6290 6265 6457 203.33 3.1% Sample 2 6502 6771 6290 6265 6457 203.33 3.1% Sample 3 26206 23293 23327 24983 24452 1221.41 4.9% Sample 3 26206 23293 23327 24983 24452 1221.41 4.9%

Gene Name

CES1

Gene ID

1066

UniProt

P23141

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G18-L567

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1504 6502 26206 1649 6732 27351 Standard deviation 102.27 435.63 1205.47 113.78 484.7 1723.11 CV (%) 6.8% 6.7% 4.6% 6.9% 7.2% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1504 6502 26206 1649 6732 27351 Standard deviation 102.27 435.63 1205.47 113.78 484.7 1723.11 CV (%) 6.8% 6.7% 4.6% 6.9% 7.2% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1504 6502 26206 1649 6732 27351 Mean (pg/ml) 1504 6502 26206 1649 6732 27351 Standard deviation 102.27 435.63 1205.47 113.78 484.7 1723.11 Standard deviation 102.27 435.63 1205.47 113.78 484.7 1723.11 CV (%) 6.8% 6.7% 4.6% 6.9% 7.2% 6.3% CV (%) 6.8% 6.7% 4.6% 6.9% 7.2% 6.3%

Sensitivity

<0.1 ng/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CES1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.005 0.093 0.154 0.281 0.561 0.934 1.358 2.129 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.005 0.093 0.154 0.281 0.561 0.934 1.358 2.129 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.005 0.093 0.154 0.281 0.561 0.934 1.358 2.129 O.D. 0.005 0.093 0.154 0.281 0.561 0.934 1.358 2.129

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.