Human CFHR5 PicoKine® Quick ELISA Kit

Complement factor H-related protein 5 is a protein that in humans is encoded by the CFHR5 gene. It is mapped to 1q31.3. This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS) .

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1228 1345 1463 1512 1387 81.83 5.9% Sample 2 5795 6654 5963 5794 6052 387.3 6.4% Sample 3 26491 29746 25641 26758 27272 1472.66 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1228 1345 1463 1512 1387 81.83 5.9% Sample 1 1228 1345 1463 1512 1387 81.83 5.9% Sample 2 5795 6654 5963 5794 6052 387.3 6.4% Sample 2 5795 6654 5963 5794 6052 387.3 6.4% Sample 3 26491 29746 25641 26758 27272 1472.66 5.4% Sample 3 26491 29746 25641 26758 27272 1472.66 5.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1228 5795 26941 1325 6341 26498 Standard deviation 81.05 376.68 1373.99 84.8 374.12 1563.38 CV (%) 6.6% 6.5% 5.1% 6.4% 5.9% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1228 5795 26941 1325 6341 26498 Standard deviation 81.05 376.68 1373.99 84.8 374.12 1563.38 CV (%) 6.6% 6.5% 5.1% 6.4% 5.9% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1228 5795 26941 1325 6341 26498 Mean (pg/ml) 1228 5795 26941 1325 6341 26498 Standard deviation 81.05 376.68 1373.99 84.8 374.12 1563.38 Standard deviation 81.05 376.68 1373.99 84.8 374.12 1563.38 CV (%) 6.6% 6.5% 5.1% 6.4% 5.9% 5.9% CV (%) 6.6% 6.5% 5.1% 6.4% 5.9% 5.9%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.028 0.269 0.470 0.722 1.123 1.623 1.985 2.387 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.028 0.269 0.470 0.722 1.123 1.623 1.985 2.387 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.028 0.269 0.470 0.722 1.123 1.623 1.985 2.387 O.D. 0.028 0.269 0.470 0.722 1.123 1.623 1.985 2.387

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.