Human COMP/Thrombospondin 5 PicoKine® Quick ELISA Kit

Human COMP/Thrombospondin 5 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human COMP/Thrombospondin 5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 257 303 298 273 283 11.03 3.9% Sample 2 1962 2068 2165 2214 2102 58.86 2.8% Sample 3 4987 4856 4713 4651 4802 259.29 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 257 303 298 273 283 11.03 3.9% Sample 1 257 303 298 273 283 11.03 3.9% Sample 2 1962 2068 2165 2214 2102 58.86 2.8% Sample 2 1962 2068 2165 2214 2102 58.86 2.8% Sample 3 4987 4856 4713 4651 4802 259.29 5.4% Sample 3 4987 4856 4713 4651 4802 259.29 5.4%

Gene Name

COMP

Gene ID

1311

UniProt

P49747

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-A757

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 257 1962 4987 269 1888 4741 Standard deviation 13.62 107.91 204.47 15.06 109.5 279.72 CV (%) 5.3% 5.5% 4.1% 5.6% 5.8% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 257 1962 4987 269 1888 4741 Standard deviation 13.62 107.91 204.47 15.06 109.5 279.72 CV (%) 5.3% 5.5% 4.1% 5.6% 5.8% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 257 1962 4987 269 1888 4741 Mean (pg/ml) 257 1962 4987 269 1888 4741 Standard deviation 13.62 107.91 204.47 15.06 109.5 279.72 Standard deviation 13.62 107.91 204.47 15.06 109.5 279.72 CV (%) 5.3% 5.5% 4.1% 5.6% 5.8% 5.9% CV (%) 5.3% 5.5% 4.1% 5.6% 5.8% 5.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human COMP

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.051 0.104 0.136 0.209 0.352 0.611 1.059 1.804 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.051 0.104 0.136 0.209 0.352 0.611 1.059 1.804 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.051 0.104 0.136 0.209 0.352 0.611 1.059 1.804 O.D. 0.051 0.104 0.136 0.209 0.352 0.611 1.059 1.804

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.