Human Chitinase 3-Like 1/YKL-40 PicoKine® Quick ELISA Kit

Human Chitinase 3-Like 1/YKL-40 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human Chitinase 3-Like 1/YKL-40 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 125 116 135 118 124 7.53 6.1% Sample 2 487 475 488 471 480 25.93 5.4% Sample 3 2196 2424 2016 1974 2153 99.02 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 125 116 135 118 124 7.53 6.1% Sample 1 125 116 135 118 124 7.53 6.1% Sample 2 487 475 488 471 480 25.93 5.4% Sample 2 487 475 488 471 480 25.93 5.4% Sample 3 2196 2424 2016 1974 2153 99.02 4.6% Sample 3 2196 2424 2016 1974 2153 99.02 4.6%

Gene Name

CHI3L1

Gene ID

1116

UniProt

P36222

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y22-T383

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 125 487 2196 117 486 2169 Standard deviation 6.75 24.84 107.6 7.25 33.05 127.97 CV (%) 5.4% 5.1% 4.9% 6.2% 6.8% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 125 487 2196 117 486 2169 Standard deviation 6.75 24.84 107.6 7.25 33.05 127.97 CV (%) 5.4% 5.1% 4.9% 6.2% 6.8% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 125 487 2196 117 486 2169 Mean (pg/ml) 125 487 2196 117 486 2169 Standard deviation 6.75 24.84 107.6 7.25 33.05 127.97 Standard deviation 6.75 24.84 107.6 7.25 33.05 127.97 CV (%) 5.4% 5.1% 4.9% 6.2% 6.8% 5.9% CV (%) 5.4% 5.1% 4.9% 6.2% 6.8% 5.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CHI3L1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.038 0.184 0.286 0.532 0.957 1.577 2.178 2.508 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.038 0.184 0.286 0.532 0.957 1.577 2.178 2.508 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.038 0.184 0.286 0.532 0.957 1.577 2.178 2.508 O.D. 0.038 0.184 0.286 0.532 0.957 1.577 2.178 2.508

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.