Human Chemerin/RARRES2 PicoKine® Quick ELISA Kit

Human Chemerin/RARRES2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human Chemerin/RARRES2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1 (also known as ChemR23) . It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 118 122 103 115 115 6.76 5.9% Sample 2 754 766 687 729 734 27.16 3.7% Sample 3 3266 2789 2969 3381 3101 192.28 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 118 122 103 115 115 6.76 5.9% Sample 1 118 122 103 115 115 6.76 5.9% Sample 2 754 766 687 729 734 27.16 3.7% Sample 2 754 766 687 729 734 27.16 3.7% Sample 3 3266 2789 2969 3381 3101 192.28 6.2% Sample 3 3266 2789 2969 3381 3101 192.28 6.2%

Gene Name

RARRES2

Gene ID

5919

UniProt

Q99969

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V17-S163

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 118 754 3266 128 779 3354 Standard deviation 6.49 47.5 192.69 6.66 57.65 197.89 CV (%) 5.5% 6.3% 5.9% 5.2% 7.4% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 118 754 3266 128 779 3354 Standard deviation 6.49 47.5 192.69 6.66 57.65 197.89 CV (%) 5.5% 6.3% 5.9% 5.2% 7.4% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 118 754 3266 128 779 3354 Mean (pg/ml) 118 754 3266 128 779 3354 Standard deviation 6.49 47.5 192.69 6.66 57.65 197.89 Standard deviation 6.49 47.5 192.69 6.66 57.65 197.89 CV (%) 5.5% 6.3% 5.9% 5.2% 7.4% 5.9% CV (%) 5.5% 6.3% 5.9% 5.2% 7.4% 5.9%

Sensitivity

<20 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human RARRES2

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.013 0.119 0.195 0.356 0.623 1.012 1.389 1.802 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.013 0.119 0.195 0.356 0.623 1.012 1.389 1.802 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.013 0.119 0.195 0.356 0.623 1.012 1.389 1.802 O.D. 0.013 0.119 0.195 0.356 0.623 1.012 1.389 1.802

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.