Mouse Chitinase 3-Like 1/YKL-40 PicoKine® Quick ELISA Kit

Mouse Chitinase 3-Like 1/YKL-40 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Chitinase 3-Like 1/YKL-40 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 112 130 114 120 7.29 6.1% Sample 2 481 475 486 451 473 26.03 5.5% Sample 3 2102 2415 2048 1889 2114 95.11 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 112 130 114 120 7.29 6.1% Sample 1 122 112 130 114 120 7.29 6.1% Sample 2 481 475 486 451 473 26.03 5.5% Sample 2 481 475 486 451 473 26.03 5.5% Sample 3 2102 2415 2048 1889 2114 95.11 4.5% Sample 3 2102 2415 2048 1889 2114 95.11 4.5%

Gene Name

CHI3L1

Gene ID

12654

UniProt

Q61362

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y22-A381

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 481 2102 115 474 2166 Standard deviation 8.17 26.94 96.69 8.17 32.23 99.64 CV (%) 6.7% 5.6% 4.6% 7.1% 6.8% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 481 2102 115 474 2166 Standard deviation 8.17 26.94 96.69 8.17 32.23 99.64 CV (%) 6.7% 5.6% 4.6% 7.1% 6.8% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 122 481 2102 115 474 2166 Mean (pg/ml) 122 481 2102 115 474 2166 Standard deviation 8.17 26.94 96.69 8.17 32.23 99.64 Standard deviation 8.17 26.94 96.69 8.17 32.23 99.64 CV (%) 6.7% 5.6% 4.6% 7.1% 6.8% 4.6% CV (%) 6.7% 5.6% 4.6% 7.1% 6.8% 4.6%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Chi3l1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.039 0.084 0.128 0.225 0.414 0.769 1.345 2.160 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.039 0.084 0.128 0.225 0.414 0.769 1.345 2.160 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.039 0.084 0.128 0.225 0.414 0.769 1.345 2.160 O.D. 0.039 0.084 0.128 0.225 0.414 0.769 1.345 2.160

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.