Mouse CXCL1/Gro Alpha PicoKine® Quick ELISA Kit

Mouse CXCL1/Gro Alpha PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse CXCL1/Gro Alpha in cell culture supernatants and serum. Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha) . In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 27 33 35 31 1.6 5.2% Sample 2 242 257 288 302 272 17.15 6.3% Sample 3 667 658 691 678 674 23.57 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 27 33 35 31 1.6 5.2% Sample 1 28 27 33 35 31 1.6 5.2% Sample 2 242 257 288 302 272 17.15 6.3% Sample 2 242 257 288 302 272 17.15 6.3% Sample 3 667 658 691 678 674 23.57 3.5% Sample 3 667 658 691 678 674 23.57 3.5%

Gene Name

Cxcl1

Gene ID

14825

UniProt

P12850

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R20-K96

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 242 667 29 264 680 Standard deviation 2.35 15.97 45.36 2.09 17.16 44.2 CV (%) 8.4% 6.6% 6.8% 7.2% 6.5% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 242 667 29 264 680 Standard deviation 2.35 15.97 45.36 2.09 17.16 44.2 CV (%) 8.4% 6.6% 6.8% 7.2% 6.5% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 242 667 29 264 680 Mean (pg/ml) 28 242 667 29 264 680 Standard deviation 2.35 15.97 45.36 2.09 17.16 44.2 Standard deviation 2.35 15.97 45.36 2.09 17.16 44.2 CV (%) 8.4% 6.6% 6.8% 7.2% 6.5% 6.5% CV (%) 8.4% 6.6% 6.8% 7.2% 6.5% 6.5%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Cxcl1

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.057 0.134 0.183 0.307 0.537 0.945 1.668 2.334 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.057 0.134 0.183 0.307 0.537 0.945 1.668 2.334 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.057 0.134 0.183 0.307 0.537 0.945 1.668 2.334 O.D. 0.057 0.134 0.183 0.307 0.537 0.945 1.668 2.334

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.