Rat CXCL1/Gro Alpha PicoKine® Quick ELISA Kit

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha) . In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 25 23 41 35 31 1.67 5.4% Sample 2 171 180 188 202 185 5.93 3.2% Sample 3 426 498 502 514 485 15.52 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 25 23 41 35 31 1.67 5.4% Sample 1 25 23 41 35 31 1.67 5.4% Sample 2 171 180 188 202 185 5.93 3.2% Sample 2 171 180 188 202 185 5.93 3.2% Sample 3 426 498 502 514 485 15.52 3.2% Sample 3 426 498 502 514 485 15.52 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 25 171 426 22 156 411 Standard deviation 1.35 10.77 19.17 1.36 9.05 28.36 CV (%) 5.4% 6.3% 4.5% 6.2% 5.8% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 25 171 426 22 156 411 Standard deviation 1.35 10.77 19.17 1.36 9.05 28.36 CV (%) 5.4% 6.3% 4.5% 6.2% 5.8% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 25 171 426 22 156 411 Mean (pg/ml) 25 171 426 22 156 411 Standard deviation 1.35 10.77 19.17 1.36 9.05 28.36 Standard deviation 1.35 10.77 19.17 1.36 9.05 28.36 CV (%) 5.4% 6.3% 4.5% 6.2% 5.8% 6.9% CV (%) 5.4% 6.3% 4.5% 6.2% 5.8% 6.9%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.035 0.124 0.201 0.358 0.640 1.121 1.863 2.448 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.035 0.124 0.201 0.358 0.640 1.121 1.863 2.448 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.035 0.124 0.201 0.358 0.640 1.121 1.863 2.448 O.D. 0.035 0.124 0.201 0.358 0.640 1.121 1.863 2.448

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.