Mouse CTLA4 PicoKine® Quick ELISA Kit

Mouse CTLA4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse CTLA4 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152 (Cluster of differentiation 152), is a protein receptor that down regulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 91 95 99 93 3.08 3.3% Sample 2 302 299 240 274 279 13.66 4.9% Sample 3 874 726 864 791 814 42.32 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 91 95 99 93 3.08 3.3% Sample 1 88 91 95 99 93 3.08 3.3% Sample 2 302 299 240 274 279 13.66 4.9% Sample 2 302 299 240 274 279 13.66 4.9% Sample 3 874 726 864 791 814 42.32 5.2% Sample 3 874 726 864 791 814 42.32 5.2%

Gene Name

Ctla4

Gene ID

12477

UniProt

P09793

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A37-F162

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 302 874 79 274 826 Standard deviation 4.84 20.84 41.95 5.21 16.17 58.65 CV (%) 5.5% 6.9% 4.8% 6.6% 5.9% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 302 874 79 274 826 Standard deviation 4.84 20.84 41.95 5.21 16.17 58.65 CV (%) 5.5% 6.9% 4.8% 6.6% 5.9% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88 302 874 79 274 826 Mean (pg/ml) 88 302 874 79 274 826 Standard deviation 4.84 20.84 41.95 5.21 16.17 58.65 Standard deviation 4.84 20.84 41.95 5.21 16.17 58.65 CV (%) 5.5% 6.9% 4.8% 6.6% 5.9% 7.1% CV (%) 5.5% 6.9% 4.8% 6.6% 5.9% 7.1%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Ctla4

Anticoagulant

Heparin

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.016 0.129 0.228 0.392 0.726 1.301 1.978 2.589 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.016 0.129 0.228 0.392 0.726 1.301 1.978 2.589 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.016 0.129 0.228 0.392 0.726 1.301 1.978 2.589 O.D. 0.016 0.129 0.228 0.392 0.726 1.301 1.978 2.589

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.