Human DKK-1/Dickkopf 1 PicoKine® Quick ELISA Kit

Human DKK-1/Dickkopf 1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human DKK-1/Dickkopf 1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 166 155 167 152 160 3.52 2.2% Sample 2 652 694 637 671 664 31.85 4.8% Sample 3 1518 1503 1246 1377 1411 63.5 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 166 155 167 152 160 3.52 2.2% Sample 1 166 155 167 152 160 3.52 2.2% Sample 2 652 694 637 671 664 31.85 4.8% Sample 2 652 694 637 671 664 31.85 4.8% Sample 3 1518 1503 1246 1377 1411 63.5 4.5% Sample 3 1518 1503 1246 1377 1411 63.5 4.5%

Gene Name

DKK1

Gene ID

22943

UniProt

O94907

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T32-H266

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 166 652 1518 147 564 1364 Standard deviation 9.79 29.34 103.224 8.67 25.38 92.75 CV (%) 5.9% 4.5% 6.8% 5.8% 4.5% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 166 652 1518 147 564 1364 Standard deviation 9.79 29.34 103.224 8.67 25.38 92.75 CV (%) 5.9% 4.5% 6.8% 5.8% 4.5% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 166 652 1518 147 564 1364 Mean (pg/ml) 166 652 1518 147 564 1364 Standard deviation 9.79 29.34 103.224 8.67 25.38 92.75 Standard deviation 9.79 29.34 103.224 8.67 25.38 92.75 CV (%) 5.9% 4.5% 6.8% 5.8% 4.5% 6.7% CV (%) 5.9% 4.5% 6.8% 5.8% 4.5% 6.7%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human DKK1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.033 0.062 0.171 0.253 0.425 0.733 1.291 1.902 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.033 0.062 0.171 0.253 0.425 0.733 1.291 1.902 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.033 0.062 0.171 0.253 0.425 0.733 1.291 1.902 O.D. 0.033 0.062 0.171 0.253 0.425 0.733 1.291 1.902

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.