Human CD26/DPP4 PicoKine® Quick ELISA Kit

Human CD26/DPP4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CD26/DPP4 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 482 550 492 487 503 30.67 6.1% Sample 2 2761 2826 2924 2841 2838 76.63 2.7% Sample 3 8848 9902 8546 9412 9177 504.74 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 482 550 492 487 503 30.67 6.1% Sample 1 482 550 492 487 503 30.67 6.1% Sample 2 2761 2826 2924 2841 2838 76.63 2.7% Sample 2 2761 2826 2924 2841 2838 76.63 2.7% Sample 3 8848 9902 8546 9412 9177 504.74 5.5% Sample 3 8848 9902 8546 9412 9177 504.74 5.5%

Gene Name

DPP4

Gene ID

1803

UniProt

P27487

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D34-P766

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 482 2761 8848 497 2879 8816 Standard deviation 32.78 124.25 575.12 29.32 155.47 696.46 CV (%) 6.8% 4.5% 6.5% 5.9% 5.4% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 482 2761 8848 497 2879 8816 Standard deviation 32.78 124.25 575.12 29.32 155.47 696.46 CV (%) 6.8% 4.5% 6.5% 5.9% 5.4% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 482 2761 8848 497 2879 8816 Mean (pg/ml) 482 2761 8848 497 2879 8816 Standard deviation 32.78 124.25 575.12 29.32 155.47 696.46 Standard deviation 32.78 124.25 575.12 29.32 155.47 696.46 CV (%) 6.8% 4.5% 6.5% 5.9% 5.4% 7.9% CV (%) 6.8% 4.5% 6.5% 5.9% 5.4% 7.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human DPP4

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.017 0.123 0.217 0.396 0.769 1.356 1.966 2.396 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.017 0.123 0.217 0.396 0.769 1.356 1.966 2.396 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.017 0.123 0.217 0.396 0.769 1.356 1.966 2.396 O.D. 0.017 0.123 0.217 0.396 0.769 1.356 1.966 2.396

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.