Human CD26/DPP4 PicoKine® Quick ELISA Kit

Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 482 550 492 487 503 30.67 6.1% Sample 2 2761 2826 2924 2841 2838 76.63 2.7% Sample 3 8848 9902 8546 9412 9177 504.74 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 482 550 492 487 503 30.67 6.1% Sample 1 482 550 492 487 503 30.67 6.1% Sample 2 2761 2826 2924 2841 2838 76.63 2.7% Sample 2 2761 2826 2924 2841 2838 76.63 2.7% Sample 3 8848 9902 8546 9412 9177 504.74 5.5% Sample 3 8848 9902 8546 9412 9177 504.74 5.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 482 2761 8848 497 2879 8816 Standard deviation 32.78 124.25 575.12 29.32 155.47 696.46 CV (%) 6.8% 4.5% 6.5% 5.9% 5.4% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 482 2761 8848 497 2879 8816 Standard deviation 32.78 124.25 575.12 29.32 155.47 696.46 CV (%) 6.8% 4.5% 6.5% 5.9% 5.4% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 482 2761 8848 497 2879 8816 Mean (pg/ml) 482 2761 8848 497 2879 8816 Standard deviation 32.78 124.25 575.12 29.32 155.47 696.46 Standard deviation 32.78 124.25 575.12 29.32 155.47 696.46 CV (%) 6.8% 4.5% 6.5% 5.9% 5.4% 7.9% CV (%) 6.8% 4.5% 6.5% 5.9% 5.4% 7.9%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.017 0.123 0.217 0.396 0.769 1.356 1.966 2.396 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.017 0.123 0.217 0.396 0.769 1.356 1.966 2.396 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.017 0.123 0.217 0.396 0.769 1.356 1.966 2.396 O.D. 0.017 0.123 0.217 0.396 0.769 1.356 1.966 2.396

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.