Human CXCL13/BLC/BCA1 PicoKine® Quick ELISA Kit

C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 41 38 34 37 2.57 6.9% Sample 2 186 197 198 189 193 5.2 2.7% Sample 3 437 495 459 516 477 11.44 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 41 38 34 37 2.57 6.9% Sample 1 36 41 38 34 37 2.57 6.9% Sample 2 186 197 198 189 193 5.2 2.7% Sample 2 186 197 198 189 193 5.2 2.7% Sample 3 437 495 459 516 477 11.44 2.4% Sample 3 437 495 459 516 477 11.44 2.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 437 34 191 423 Standard deviation 2.19 9.67 28.84 2.1 12.6 33.41 CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 437 34 191 423 Standard deviation 2.19 9.67 28.84 2.1 12.6 33.41 CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 437 34 191 423 Mean (pg/ml) 36 186 437 34 191 423 Standard deviation 2.19 9.67 28.84 2.1 12.6 33.41 Standard deviation 2.19 9.67 28.84 2.1 12.6 33.41 CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9% CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.052 0.140 0.187 0.311 0.516 0.916 1.448 2.196 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.052 0.140 0.187 0.311 0.516 0.916 1.448 2.196 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.052 0.140 0.187 0.311 0.516 0.916 1.448 2.196 O.D. 0.052 0.140 0.187 0.311 0.516 0.916 1.448 2.196

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.