Mouse CXCL16 PicoKine® Quick ELISA Kit

Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha.2 The gene for human CXCL16 is located on chromosome 17p13. The standard product used in this kit is recombinant mouse CXCL16, consisting of 88 amino acids with the molecular mass of 9.9KDa.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 34 35 37 36 2.29 6.3% Sample 2 179 204 186 197 191 9.65 5% Sample 3 538 463 494 471 491 29.15 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 34 35 37 36 2.29 6.3% Sample 1 40 34 35 37 36 2.29 6.3% Sample 2 179 204 186 197 191 9.65 5% Sample 2 179 204 186 197 191 9.65 5% Sample 3 538 463 494 471 491 29.15 5.9% Sample 3 538 463 494 471 491 29.15 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 179 538 40 171 574 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 179 538 40 171 574 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 179 538 40 171 574 Mean (pg/ml) 40 179 538 40 171 574 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5% CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.016 0.187 0.329 0.600 1.058 1.774 2.276 2.405 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.016 0.187 0.329 0.600 1.058 1.774 2.276 2.405 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.016 0.187 0.329 0.600 1.058 1.774 2.276 2.405 O.D. 0.016 0.187 0.329 0.600 1.058 1.774 2.276 2.405

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.